全文获取类型
收费全文 | 979篇 |
免费 | 77篇 |
国内免费 | 44篇 |
出版年
2023年 | 25篇 |
2022年 | 20篇 |
2021年 | 53篇 |
2020年 | 40篇 |
2019年 | 56篇 |
2018年 | 52篇 |
2017年 | 23篇 |
2016年 | 33篇 |
2015年 | 46篇 |
2014年 | 41篇 |
2013年 | 64篇 |
2012年 | 34篇 |
2011年 | 34篇 |
2010年 | 22篇 |
2009年 | 43篇 |
2008年 | 57篇 |
2007年 | 36篇 |
2006年 | 31篇 |
2005年 | 29篇 |
2004年 | 18篇 |
2003年 | 32篇 |
2002年 | 21篇 |
2001年 | 17篇 |
2000年 | 11篇 |
1999年 | 13篇 |
1998年 | 15篇 |
1997年 | 16篇 |
1996年 | 15篇 |
1995年 | 25篇 |
1994年 | 17篇 |
1993年 | 22篇 |
1992年 | 16篇 |
1991年 | 9篇 |
1990年 | 11篇 |
1989年 | 14篇 |
1988年 | 11篇 |
1987年 | 8篇 |
1986年 | 5篇 |
1985年 | 6篇 |
1984年 | 12篇 |
1983年 | 4篇 |
1982年 | 4篇 |
1981年 | 7篇 |
1980年 | 5篇 |
1979年 | 7篇 |
1977年 | 4篇 |
1976年 | 4篇 |
1974年 | 3篇 |
1973年 | 3篇 |
1972年 | 3篇 |
排序方式: 共有1100条查询结果,搜索用时 31 毫秒
51.
52.
Francesca Giampieri Md Soriful Islam Stefania Greco Massimiliano Gasparrini Tamara Y. Forbes Hernandez Giovanni Delli Carpini Stefano Raffaele Giannubilo Andrea Ciavattini Bruno Mezzetti Luca Mazzoni Franco Capocasa Mario Castellucci Maurizio Battino Pasquapina Ciarmela 《Journal of cellular physiology》2019,234(5):7622-7633
Uterine leiom yomas are benign tumors highly prevalent in reproductive women. In thecurrent study, initially, we aimed to screen five different strawberry cultivars (Alba, Clery, Portola, Tecla, and Romina) to identify efficient cultivars in terms of phytochemical characterization and biological properties by measuring phenolic and anthocyanin content as well as antioxidant capacity, and by measuring apoptotic rate and reactive oxygen species (ROS) production in uterine leiomyoma cells. Next, we focused on the most efficient ones, cultivar Alba (A) and Romina (R) as well as Romina anthocyanin (RA) fraction for their ability to regulate oxidative phosphorylation (oxygen consumption rate [OCR]) glycolysis (extracellular acidification rate [ECAR]), and also fibrosis. Leiomyoma and myometrial cells were treated with a methanolic extract of A and R (250 μg/ml) or with RA (50 μg/ml) for 48 hr to measure OCR and ECAR, as well as gene expression associated with fibrosis. In the leiomyoma cells, RA was more effective in inducing apoptosis and increasing intracellular ROS levels, followed by R and A. In myometrial cells, all strawberry treatments increased the cellular viability and decreased ROS concentrations. Leiomyoma cells showed also a significant decrease in ECAR, especially after RA treatment, while OCR was slightly increased in both myometrial and leiomyoma cells. R and RA treatment significantly decreased collagen 1A1, fibronectin, versican, and activin A messenger RNA expression in leiomyoma cells. In conclusion, this study suggests that Romina, or its anthocyanin fraction, can be developed as a therapeutic and/or preventive agent for uterine leiomyomas, confirming the healthy effects exerted by these fruits and their bioactive compounds. 相似文献
53.
Liang Wang Jin Sun Pei Gao Ke Su Huanhuan Wu Junli Li Weihua Lou 《Journal of cellular physiology》2019,234(9):15941-15950
Wnt1-inducible signaling protein 1 (WISP1) is a matricellular protein and downstream target of Wnt/β-catenin signaling. This study sought to determine the role of WISP1 in glucose metabolism and chemoresistance in laryngeal squamous cell carcinoma. WISP1 expression was silenced or upregulated in Hep-2 cells by the transfection of WISP1 siRNA or AdWISP1 vector. Ectopic WISP1 expression regulated glucose uptake and lactate production in Hep-2 cells. Subsequently, the expression of glucose transporter 1 (GLUT1) was significantly modulated by WISP1. Furthermore, WISP1 increased cell survival rates, diminished cell death rates, and suppressed ataxia-telangiectasia-mutated (ATM)-mediated DNA damage response pathway in cancer cells treated with cisplatin through GLUT1. WISP1 also promoted cancer cell tumorigenicity and growth in mice implanted with Hep-2 cells. Additionally, WISP1 activated the YAP1/TEAD1 pathway that consequently contributed to the regulation of GLUT1 expression. In summary, WISP1 regulated glucose metabolism and cisplatin resistance in laryngeal cancer by regulating GLUT1 expression. WISP1 may be used as a potential therapeutic target for laryngeal cancer. 相似文献
54.
Lixin Zhang Cui Ma Xiaoying Wang Siyu He Qian Li Yutian Zhou Ying Liu Min Zhang Xiufeng Yu Xijuan Zhao Fei Li Da-ling Zhu 《Journal of cellular physiology》2019,234(6):9255-9263
Abnormal airway smooth muscle cells (ASMCs) proliferation is an important pathological process in airway remodeling contributes to increased mortality in asthma. Mitochondrial dynamics and metabolism have a central role in the maintenance of the cell function. In this study, lipopolysaccharide (LPS)-induced ASMCs proliferative model was used to investigate the effect of mitochondria on the proliferation of ASMCs and the possible mechanism. We used cell and molecular biology to determine the effect of dynamin-related protein 1 (Drp1) on LPS-mediated ASMCs cell cycle progression and glycolysis. The major findings of the current study are as follows: LPS promoted an increased mitochondrial fission and phosphorylation of Drp1 at Ser616 (p-Drp1 Ser616). LPS-induced ASMCs proliferation and cell cycle progression, which was significantly inhibited application of Drp1 RNA interfering. Glycolysis inhibitor 2-deoxyglucose (2-DG) depressed ASMCs proliferative process induced by LPS stimulation. LPS caused mitochondrial metabolism disorders and aerobic glycolysis in a dependent on Drp1 activation. These results indicated that Drp1 may function as a key factor in asthma airway remodeling by mediating ASMC proliferation and cell cycle acceleration through an effect on mitochondrial metabolic disturbance. 相似文献
55.
Jiliang Yan Dongya Zhang Yanjie Han Zhongjie Wang Chunyan Ma 《Journal of cellular biochemistry》2019,120(10):17303-17311
SR splicing-factors (SRSFs) play a vital role in carcinogenesis. SRSF5 was demonstrated to be upregulated in lung cancer and identified as a novel prognostic indicator for small-cell lung cancer. However, the role of SRSF5 in the pathogenesis of non–small cell lung cancer (NSCLC) and the molecular mechanism involved are still undefined. The expression of SRSF5 in NSCLC cells was detected by quantitative real-time polymerase chain reaction and Western blot analysis. The proliferation of cells was evaluated by cell counting kit-8 and BrdU assays. Apoptosis was assessed by flow cytometry and Western blot analysis of apoptosis-associated proteins including B-cell lymphoma 2 (Bcl-2), Bax, and cytochrome C (Cyt C). Glycolysis was detected by determining glucose consumption, lactate production, and pyruvate kinase M2 (PKM2) expression. We found that SRSF5 messenger RNA and protein levels were elevated in NSCLC cells. SRSF5 knockdown inhibited the proliferation and Ki67 expression in NSCLC cells. SRSF5 silencing increased the apoptotic rate, upregulated Bax and Cyt C, and decreased Bcl-2 level in NSCLC cells. Moreover, Knockdown of SRSF5 repressed glycolysis in NSCLC cells via reducing PKM2 expression. Enhanced glycolysis by PKM2 overexpression attenuated the effects of SRSF5 silencing on NSCLC cell proliferation and apoptosis. Overall, knockdown of SRSF5 inhibited proliferative ability and induced apoptosis by suppressing PKM2 expression in NSCLC cells. 相似文献
56.
Degradation of indole by enrichment culture and Pseudomonas aeruginosa Gs isolated from mangrove sediment 总被引:1,自引:1,他引:0
Microbial degradation of indole was investigated using enrichment culture and Pseudomonas aeruginosa Gs obtained from mangrove sediment of Hong Kong Nature Reserve as inocula. Degradation of indole using this enrichment culture and Ps. aeruginosa Gs was quantified on reversed-phase high-performance liquid chromatography. Initial concentrations of indole affected degradation and the results conformed to the zero-order kinetic model. The optimum pH and salinity were 7.0 and 5‰, respectively, for indole degradation by Ps. aeruginosa Gs. Two major metabolites of indole degradation were detected. This study suggests that indole can be rapidly degraded by indigenous microorganisms of the mangrove environment. 相似文献
57.
Mingshan Xue Yifeng Zeng Runpei Lin Hui-Qi Qu Teng Zhang Xiaohua Douglas Zhang Yueting Liang Yingjie Zhen Hao Chen Zhifeng Huang Haisheng Hu Peiyan Zheng Hakon Hakonarson Luqian Zhou Baoqing Sun 《Experimental biology and medicine (Maywood, N.J.)》2021,246(14):1586
While there is no cure for chronic obstructive pulmonary disease (COPD), its progressive nature and the formidable challenge to manage its symptoms warrant a more extensive study of the pathogenesis and related mechanisms. A new emphasis on COPD study is the change of energy metabolism. For the first time, this study investigated the anaerobic and aerobic energy metabolic pathways in COPD using the metabolomic approach. Metabolomic analysis was used to investigate energy metabolites in 140 COPD patients. The significance of energy metabolism in COPD was comprehensively explored by the Global Initiative for Chronic Obstructive Lung Disease–GOLD grading, acute exacerbation vs. stable phase (either clinical stability or four-week stable phase), age group, smoking index, lung function, and COPD Assessment Test (CAT) score. Through comprehensive evaluation, we found that COPD patients have a significant imbalance in the aerobic and anaerobic energy metabolisms in resting state, and a high tendency of anaerobic energy supply mechanism that correlates positively with disease progression. This study highlighted the significance of anaerobic and low-efficiency energy supply pathways in lung injury and linked it to the energy-inflammation-lung ventilatory function and the motion limitation mechanism in COPD patients, which implies a novel therapeutic direction for this devastating disease. 相似文献
58.
A reconstituted glycolytic system has been established from individually purified enzymes to simulate the conversion of glucose to ethanol plus CO2 by yeast. Sustained and extensive conversion occurred provided that input of glucose matched the rate of ATP degradation appropriately.ATPase activity could be replaced by arsenate, which uncoupled ATP synthesis from glycolysis. The mode of uncoupling was investigated, and it was concluded that the artificial intermediate, 1-arseno-3-phosphoglycerate, has a half-life of no more than a few milliseconds. Arsenate at 4 mM concentration could simulate the equivalent of 10 μmol ml?1 min?1 of ATPase activity.The reconstituted enzyme system was capable of totally degrading 1 M (18% w/v) glucose in 8 h giving 9% (w/v) ethanol. The levels of metabolites during metabolism were measured to detect rate-limiting steps.The successful operation of the reconstituted enzyme system demonstrates that it is possible to carry out complex chemical transformations with multiple enzyme systems in vitro. 相似文献
59.
Sàágua M.C. Baeta-Hall L. Anselmo A.M. 《World journal of microbiology & biotechnology》2002,18(9):841-845
The soil microbial population of a coke oven site was investigated in order to evaluate its potential for bioremediation. The study was carried out in soil samples with distinct polynuclear aromatic hydrocarbon (PAH) contamination levels, comparing the population profiles constituted by total heterotrophic and PAH-utilizing strains. Isolation of degrading strains was performed with phenanthrene or pyrene as sole carbon sources. The ability to degrade other PAHs, such as anthracene, fluorene and fluoranthene was also investigated. The results showed a reduction of 30% in species diversity and microbial density drops one order of magnitude in contaminated samples. Furthermore, the number of PAH-utilizing colonies was higher in the contaminated area and about 20% of the isolates were able to degrade phenanthrene and pyrene, while this value decreased to 0.15% in uncontaminated samples. Three PAH-degrader strains were identified as: CDC gr. IV C-2, Aeromonas sp. and Pseudomonas vesicularis. The ability of these strains to degrade other PAHs was also investigated. 相似文献
60.
A. Westram J. R. Lloyd U. Roessner J. W. Riesmeier & J. Kossmann† 《Plant, cell & environment》2002,25(9):1133-1143